Here, we further demonstrated this phenomenon in the non-human primate model. Infection of macaques with the LR2006-OPY1 isolate, which encodes K 252 in the E2 glycoprotein, strongly induced anti-CHIKV antibodies against a particular linear B-cell epitope (amino acids 3025–3066) at 16 dpi. In line with this, we observed significantly stronger neutralizing activity against the IMT isolate (encoding K 252 in E2) compared to the SGP11 isolate (encoding Q 252 in E2) (). These data complement a separate study that showed stronger neutralizing antibody response in animals infected with the ECSA strain (DHS-4263) compared to animals infected with the West African strain (37997). Chepikov vt pedagogika. Paradoxically, the difference in neutralizing capacity observed here was lost in macaque sera taken at 180 dpi.

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Furius and Cossinius can't capture the rebel slaves army, nor properly keep their plans secret. Realizing hiding will be much harder in winter, Spartacus decides to capture a town.

First he and a trusted band sneak up to the commanders' hideout villa to slay them. The senate fears Rome itself may be endangered but lacks funds and men to mount a suitably large army. So Metellus turns to immensely rich, ambitious Marcus Crassus, who accepts, to eager son Tiberius's frustration without even negotiating an accordingly exalted status or mandate, and continuous private. Spartacus decides his army now needs a city to pass the winter, and lets escaped local slave Diotimos inspire his war council to choose and plan how to take over well-guarded Sinue (modern Sinuessa). Marcus Crassus completes preparing his legionary army. Eldest son Tiberius hopes his help will be rewarded with second rank, but fears being side-tracked in favor of seasoned young, general Caius Julius Caesar.

The aristocrat last needs a rich, ambitious ally to pay his debts and finance his campaign, but loses points with Marcus by cockily playing with his slave-lover. Crassus sends Tiberius ahead with instructions to observe Sinuessa and wait. Caesar won't stand for being the boy's deputy and beheads the 'cowardly' gate keeper who escaped and reports Spartacus took the city easily. Despite Spartacus's explicit instructions, surviving Roman citizens suffer excessive cruelty, especially at the hands of Crixus's traumatized Naevia, who even kills blacksmith Attius. When Cilician pirates arrive, Spartacus learns their captain Heraclea has a secret deal with the late aedilius Ennius to use his seal to sell his thus laundered loot at. Although Sinuesse's food supplies are running out and Cilican pirate captain Heracleo can't promise enough (except wine) even at premium prices, Spartacus orders to admit new slaves, after screening for undercover Romans, a group of which fails to force the gate.

Young Caius Julius Caesar manages to pose as a escaped shepherd slave and wins the confidence of dishonest schemer Nemetes. Thus he discovers and fuels dissension in Spartacus's camp, yet causes only the slaughter of most Romans and doubt in Crixus's mind whether Spartacus should still be obeyed. Tiberius seems a bitter, broken boy, having had to beat his comrade Sabinus to death, lashing out at surviving true cowards. Father Marcus Crassus sends his mistress to coax his heir with wine and tenderness, but the knave chooses to rape the 'mere' slave by way of compensation. Senator Metellus is ignored when he rudely inquires why the army hasn't moved on Sinuessa, but will soon see. Spartacus tests Cricus' loyal patience to breaking point by leaving him behind during raids on Crassus's food supplies, announces he's sailing on Heracleo's ships to Sicily. Caesar's masterly manipulation allows a triumphant Roman recapture of Sinessa.